氫氣治療糖尿病皮膚損傷的細胞學研究
氫氣保護高糖和甘露醇誘導的人皮膚成纖維細胞氧化損傷
第三軍醫大學整形外科關於氫氣治療糖尿病皮膚損傷的細胞研究，最近剛剛被BBRC接受。本研究不僅豐富了氫氣治療疾病的研究範圍，而且給糖尿病皮膚損傷的治療提供了一種新的治療希望。為人們理解氫氣對糖尿病的治療效果提供了新的證據。
活性氧引起的氧化應激是糖尿病患者皮膚損傷的重要因素，氫氣是一種剛發現的毒性活性氧（(•OH和ONOO-)）抗氧化物質，但不與其他類型的活性氧發生反應（不一定）。本研究主要觀察氫氣培養基對人皮膚成纖維細胞氧化損傷的保護作用。
採用鐳射共聚焦顯微鏡分析細胞內O2-含量和線粒體膜電位。採用CCK-8試劑檢測細胞存活率。並檢測了細胞MDA、SOD、GSH含量。結果發現，高糖和甘露醇都可以誘導的人皮膚成纖維細胞氧化損傷。有意思的是，溶解氫氣的培養基能顯著減少細胞內O2-含量，穩定線粒體膜電位，降低MDA含量，細胞抗氧化能力明顯提高。說明氫氣具有提高細胞抗氧化能力，減少活性氧產生。研究結果提示氫氣對糖尿病等各類皮膚損傷具有潛在的應用價值。
Title: Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitolinduced oxidative damage
Abstract: Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (•OH) and peroxynitrite (ONOO-) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In the present study, we investigated the protective effects of hydrogen-rich medium on human skin fibroblasts (HSFs) under oxidative stress.
Confocal microscopy was used to assay both the intracellular superoxide anion (O2-) concentration and the mitochondrial membrane potential (△Ψ). Cell viability was determined using the Cell Counting Kit-8 (CCK-8). The concentrations of cellular malonaldehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were also measured. The results revealed that both mannitol and high glucose could cause oxidative stress in HSFs. Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O2-, stabilized the △Ψ and attenuated production of MDA, which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage. In other words, hydrogen decreased the excessive generation of intracellular O2- and elevated the cellular antioxidative defense. Based on our results, hydrogen may have applications in the treatment of skin diseases caused by diabetes.
Keywords: Hydrogen, skin lesions, human skin fibroblast, reactive oxygen species, superoxide anion, mitochondrial membrane potential
原始論文：http://blog.sciencenet.cn/home.php?mod=attachment&id=5026
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